Collection of small subunit (16S- and 16S-like) ribosomal RNA structures: 1994

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Collection of small subunit (16S- and 16S-like) ribosomal RNA structures

Inferring higher-order structure for complex RNA molecules, such as the ribosomal RNAs has relied primarily on comparative methods. Underlying these methods is the premise that molecules with different primary structure and similar functional characteristics have similar secondary and tertiary structure [Reviewed in: 1]. For these methods to be effective, the RNA molecules under study need to b...

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Major efforts are underway to elucidate the spatial distribution of ribosomal RNAs (rRNAs) in the ribosome. An especially informative approach is the identification of likely base-base tertiary interactions within the RNA by phylogenetic comparison. Herein evidence is presented for three heretofore unrecognized candidate tertiary interactions, G506/C525, C779/G803 and A994/U1380 (1) in 16S-like...

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Compilation of small ribosomal subunit RNA structures

The database on small ribosomal subunit RNA structure contained 1804 nucleotide sequences on April 23, 1993. This number comprises 365 eukaryotic, 65 archaeal, 1260 bacterial, 30 plastidial, and 84 mitochondrial sequences. These are stored in the form of an alignment in order to facilitate the use of the database as input for comparative studies on higher-order structure and for reconstruction ...

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Developing artificial genetic switches in order to control gene expression via an external stimulus is an important aim in chemical and synthetic biology. Here, we expand the application range of RNA switches to the regulation of 16S rRNA function in Escherichia coli. For this purpose, we incorporated hammerhead ribozymes at several positions into orthogonalized 16S rRNA. We observed that ribos...

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The 16S ribosomal RNA mutation database (16SMDB)

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1994

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/22.17.3502